Research Supervision Capacity
About Me
Dr. Muhammad Rashid is currently working as Assistant Professor in Department of Parasitology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur. He did DVM in 2013 from University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur (IUB) and MPhil Parasitology in 2016 from University of Veterinary and Animal Sciences (UVAS), Lahore. In this year, joined Lanzhou Veterinary Research Center (LVRI), Chinese Academy of Agricultural Sciences (CAAS), China as PhD scholar. During his stay in LVRI, worked on “Impacts of Theileria annulata-transformation on the cytokine profiling of B lymphocytes and endocytosis/presentation of dendritic cells purified from cattle” to completed his doctorate degree in Veterinary Parasitology and Molecular Biology. He joined IUB as Assistant Professor in Nov. 2020. He is now actively involved in developing research activities to diagnose, drug resistance, Molecular Parasitology and innumology in addition to academic activities.
1. Mutation in Theileria annulata cytochrome b gene associated with buparvaquone resistance (1million)
2. Development of a rapid and low cost lateral flow immunoassay (LFIA) diagnostic kit for Echinococcosis (16.471million)
Research Interest Keywords
Contact Info
Research Publication
1. Yousaf, Areeba and Shahzad, Mirza Imran and Ehsan, Muhammad and Hu, Rui-Si and Shahid, Kinza and Rashid, Muhammad and Malik, Muhammad Irfan and Khan, Muhammad Islam. 2026. “Recombinant Fasciola gigantica tegumental calcium binding protein-4 modulates host peripheral blood mononuclear cell immune responses in vitro.” Elsevier BV 282. 10.1016/j.actatropica.2026.108282
2. Obaid, M. K., Yuchun, C., Shehla, S., Luo, J., Adugna, T., Khan, A, Z., Rashid, M., Ren, Q., Guan G. 2026. “Rickettsia massiliae and its public health significance across Palearctic and Oriental regions: a scoping review.” BMC 79. 10.1186/s40249-026-01456-3
3. Kinza Shahid, Mirza Imran Shahzad, Muhammad Ehsan, Rui-Si Hu, Areeba Yousaf, Gildardo Rivera, Muhammad Safdar, Muhammad Rashid, Muhammad Irfan Malik, Muhammad Mudasser Nazir. 2026. “Proteomic characterization of adult Fasciola gigantica recovered from naturally infected buffaloes in Punjab Pakistan.” PLoS One 21. https://doi.org/10.1371/journal. pone.0356497
4. Naseer, Z. F., Ehsan, M., Abbasi, F., Hu, R. S., Yousaf, A., Shahid, K., Rashid, M., Malik, M. I. 2026. “Prevalence and molecular genotyping of cystic echinococcosis among Buffalo isolates from South-Punjab, Pakistan.” Elsevier 69. 10.1016/j.vprsr.2026.101455
5. Muhammad Kashif Obaid, Jin Luo, Shuaiyang Zhao, Zhancheng Tian, Shakir Ullah, Jehan Zeb, Guangyuan Liu, Jianxun Luo, Hong Yin, Muhammad Rashid, Qiaoyun Ren , Guiquan Guan. 2025. “Molecular Profiling of Ticks and Associated Pathogens: First Report of?Rickettsia sibirica,?Rickettsia slovaca, and?Babesia microti?in Ticks From Pakistan.” Wiley 26. 10.1155/tbed/3157047
6. Obaid, M. K., Lan, X., Ren, Q., Zeb, J., Luo, J., Yang, J., Jia, W., Zan, X., Yin, H., Rashid, M., Guan, G. 2025. “Molecular insights into Rickettsiales in blood and ticks of two-humped camels at Gansu Province, China: With an accidental detection of?Colpodella?sp.” Elsevier 305. 10.1016/j.vetmic.2025.110528
7. Obaid, M. K., Ren, Q., Luo, J., Wang, J., Rashid, M., Zeb, J., Zan, X., Lan, X., Liu, G., Luo, J., Yin, H., Chen, Z., Guan, G. 2025. “Evaluation of fipronil efficacy and first molecular report of gamma-aminobutyric acid (GABA) gated chloride channel gene of Rhipicephalus microplus ticks in China and Pakistan.” Elsevier 334. 10.1016/j.vetpar.2025.110407
8. Obaid, M. K., Ren, Q., Zan, X., Luo, J., Zeb, J., Rashid, M., Liu, G., Yang, L., Yun, X., Ma, L., Ma, P., Ma, C., Yang, X., Yin, H., Guan, G. 2025. “Pathogen Profiling and Molecular Evaluation Along With In Silico Modeling of Voltage-Gated Sodium Channel Gene in Deltamethrin Exposed Haemaphysalis longicornis Ticks.” Wiley 39. 10.1096/fj.202501223RRR
9. Rashid, M., Hayat, M. H., Zahra, N., Khan, M. S., Nadeem, M., ur Rehman, T., Ehsan, M., Malik, M.I., Dilber, G.H., Bakhsh, A., Almutairi, M.M. 2024. “Systematic review on buparvaquone resistance associated with non-synonymous mutation in drug binding genes site of Theileria annulate.” Elsevier 332. 10.1016/j.vetpar.2024.110321
10. Ehsan, M., Hu, R. S., Wang, M., Hou, J. L., Rashid, M., & Malik, M. I. 2024. “Immune modulation of goat monocytes by Fasciola gigantica Legumain-1 protein (Fg-LGMN-1).” Experimental Parasitology 108671. https://doi.org/10.1016/j.exppara.2023.108671
11. Muhammad Kashif Obaid, Shehla Shehla, Guiquan Guan, Muhammad Rashid, Sumaira shams. 2024. “Genotyping of Ticks; with First Molecular Report of Hyalomma asiaticum, and Molecular Detection of Tick-Borne Bacteria in Ticks and Blood from Khyber Pakhtunkhwa, Pakistan.” Frontiers in Cellular and Infection Microbiology 14. https://doi.org/10.3389/fcimb.2024.1346595
12. Tauseef ur Rehman, Fahmy Gad Elsaid , Maria Magdalena Garijo Toledo, Arcangelo Gentile, Riaz Ahmed Gul, Muhammad Rashid, Muhammad Tahir Aleem and Muhammad Arfan Zaman. 2023. “Fasciolosis: Recent Update in Vaccines Development and Their Efficacy.” Pakistan Veterinay Journal 45. 10.29261/pakvetj/2023.034
13. Rehman TU, El-Mansi AA, Alhag SK, Al-Shuraym LA, Saeed Z, Arif M, Rashid M, Bhutta ZA and Zaman MA. 2023. “Antiparasitic activity of methanolic and ethyl acetate extracts of Azadirachta indica against Haemonchus contortus.” Pakistan Veterinay Journal 43. 10.29261/pakvetj/2023.014
14. Quratulain, S. A., Qayyum, M., Khan, I. A., Anwar, M. N., Nawaz, M., ur Rehman, T., ... & Malik, M. I. 2023. “Coccidiosis: Prevalence, Epizootiological Risk Factors, Hematological and Serum Biochemical Profile in Clinically Infected Pet Dogs.” Pak Vet J 43. http://dx.doi.org/10.29261/pakvetj/2023.113
15. Wang, Y., Zhang, S., Wang, J., Rashid, M., Wang, X., Liu, X., Yin, H., Guan, G. 2022. “Nested qPCR assay to detect Babesia duncani infection in hamsters and humans.” Parasitology Research 121. https://doi.org/10.1007/s00436-022-07685-3
16. Rashid, M., Zahra, N., Chudhary, A., Rehman, T.U., Aleem, M.T., Alouffi, A., Mohammed, A., Ehsan, M., Malik, M.I., Dilber, G.H., Bakhsh, A., Almutairi, M.M. 2022. “Cost-benefit ratio of anthelmintic treatment and its comparative efficacy in commercial dairy farms.” Frontiers in Veterinary Science 9. org/10.3389/fvets.2022.1047497
17. Madiha Sana, Muhammad Rashid, Imran Rashid, Haroon Akbar, Gomez-Marin, Jorge; Poisson, Isabelle. 2022. “Immune Response against Toxoplasmosis- Some Recent Updates.” International Journal of Immunopathology and Pharmacology 36. 10.1177/03946320221078436
18. Muhammad Uzair Mukhtat, Maria Mukhtar, Naveed Iqbal, Zeeshan Nawaz, Adil Bhatti, Fatima Haq, Ali Arslan, Muhammad Rashid. 2021. “The emergence of Dengue Fever in Sheikhupura, Pakistan: its seroprevalence and risk factors assessment during 2014-17.” The Journal of Infection in Developing Countries 15. 10.3855/jidc.13976
19. Junlong Liu, Muhammad Rashid, Jinming Wang, Aihong Liu, Guiquan Guan, Youquan Li, Lan He, Hong Yin, Jian-xun Luo. 2020. “Theileria annulata transformation altered cell surface molecules expression and endocytosis functions of monocyte-derived dendritic cells.” Tick and Tick-borne Diseases 11. 10.1016/j.ttbdis.2019.101365
20. Wenge Liu, Jin Luo, Qiayun Ren, Zhi-Qiang Qu, Han-Liang Lin, Xiao-Feng Xu, Jun Ni, Rong-Hai Xiao, Rong-Gui Chen, Muhammad Rashid, Ze-Gong Wu, Yang-Chun Tan, Xiao-Fei Qiu, Jianxun Luo, Hong Yin, Hui Wang, Zengqi Yang, Sa Xiao and Guang-Yuan Liu. 2020. “A novel miRNA—hlo-miR-2—serves as a regulatory factor that controls molting events by targeting CPR1 in Haemaphysalis longicornis nymphs.” Front. Microbiol 11. 10.3389/fmicb.2020.01098
21. Nasreen, Sadaf Niaz, Adil Khan, Sultan Ayaz, Muhammad Rashid, Irfan Khattak, Zhijun Yu, Tianhong Wang. 2020. “Molecular Characterization of Ticks Infesting Livestock in Khyber Pakhtunkhwa Province, Pakistan.” International Journal of Acarology 46. https://doi.org/10.1080/01647954.2020.1734082
22. Liu, G. Y., Liu, W., Guo, J., Luo, J., Ren Q., Ze C., Qu, Z., Wu, Z., Ni, J., Xu, X., Rashid, M., Luo, J., H, Yin. And Yang, Z. 2020. “Analysis of microRNA expression profiles dynamic in different life stages of Haemaphysalis longicornis ticks by deep sequencing of small RNA libraries.” Ticks and Tick-borne Diseases 11. https://doi.org/10.1016/j.ttbdis.2020.101427
23. Jinming Wang, Jifei Yang, Shandian Gao, Aihong Liu, Youquan Li, Muhammad Rashid, Youquan Li, Zhijie Liu, Junlong Liu, Guangyuan Liu, Jian-xun Luo, Guiquan Guan, Hong Yin. 2020. “Rapid detection and differentiation of Theileria annulata, T. orientalis and T. sinensis using high-resolution melting analysis.” Tick and Tick-borne Diseases 11. https://doi.org/10.1016/j.ttbdis.2019.101312
24. Rashid, M., Liu, J., Guan, J., Wang, J., Li, Z., Hassan, M. A., Rashid, M, I., Mukhtar, M. U., Luo, J., Yin, H. 2020. “In vitro influence of Theileria annulata on the functions of bovine dendritic cell for stimulation of T lymphocytes proliferation.” Parasitology 139. 10.1017/S0031182019001227
25. Quanying Ma, Zhi Li, Xuerong Liu, Jing Li, Muhammad Rashid, Junlong Liu, Jinming Wang, Aihong Liu, Youquan Li, Hong Yin, Guiquan Guan, Jianxun Luo. 2020. “Optimization of a suspension culture for a Theileria annulata-infected bovine cell line.” Acta Tropica 202. https://doi.org/10.1016/j.actatropica.2019.105237
26. Muhammad Uzair Mukhtar, Naveed Iqbal, Jifei Yang, Qingli Niu, Shuiyang Zhao, Zhi Li, Yaro Zhao, Muhammad Rashid, Ze Chen, Guiquan Guan, Zhijie Liu, Hong Yin. 2020. “Identification and evaluation of UL36 protein from Dermacentor silvarum salivary gland and its interaction with Anaplasma ovis VirB10.” Parasites & Vectors 13. https://doi.org/10.1186/s13071-020-3975-9
27. Hanif, U., Abdul, Q., Rashid, M., Rashid, M. I., Guo, F. 2020. “Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.” Parasitology 147. https://doi.org/10.1017/S0031182019001665
28. Jinming Wang, Aihong Liu, Shangdi Zhang, Shandian Gao, Muhammad Rashid, Youquan Li, Junlong Liu, Quanying Ma, Zhi Li, Zhijie Liu, Jianxun Luo, Guiquan Guan, Hong Yin. 2019. “High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis.” Parasites & Vectors 12. 10.1186/s13071-019-3781-4
29. Rashid, M., Guan, G., Luo, J., Zhao, S., Wang, X., Hassan, M. A., Rashid, M. I., Mukhtar, M. U., Liu, J., Yin, H. 2019. “Establishment and expression of cytokines from Theileria annulata infected bovine B cell line.” Gene 10. 10.3390/genes10050329
30. Rashid, M, H. Akbar, A. Bakhsh, M. I. Rashid, M. A. H., R. Ullah, T. Hussain, S. Manzoor, H. Yin. 2019. “Assessing the Prevalence and Economic Significance of Coccidiosis Individually and in Combination with Concurrent Infections in Pakistani Commercial Poultry Farms.” Poultry Science 98. 10.3382/ps/pey522
31. Rashid, M., Rashid, M. I., Akbar, H., Ahmad, L., Hassan, M., Ashraf, K., Saeed, K., Gharbi, M. 2019. “A systematic review on modelling approaches for economic losses studies caused by parasites and their associated diseases in cattle.” Parasitology 146. 10.1017/S0031182018001282
32. Hassan, M. A., Liu, J., Sajid, M. S., Rashid, M., Mahmood, A., Abbas, Q., Guan, G., Yin, H., Luo, J. 2018. “Simultaneous detection of Theileria annulata and Theileria orientalis infections using recombinase polymerase amplification.” Ticks and Tick-borne Diseases 9. 10.1016/j.ttbdis.2018.03.028
33. Rashid, M., Haroon, A., Rashid, M. I., Khalid, S., Liaquat, A., Saghir, A., Shehzad, W., Islam, S., Shahid, F. 2018. “Economic significance of tropical theileriosis on a Holstein Friesian dairy farm in Pakistan.” The Journal of Parasitology 104. 10.1645/16-179
34. Ali, M. S., Saeed, K., Rashid, I., Ijaz, M., Akbar, H., Rashid, M., & Ashraf, K. 2018. “Anthelmintic Drugs: Their Efficacy and Cost-Effectiveness in Different Parity Cattle.” The Journal of Parasitology 104. 10.1645/17-4
35. Muhammad Adeel Hassan, Junglong Liu, Muhammad Rashid, Naveed Iqbal, Guiquan Guan, Hong Yin, Jianxun Luo. 2018. “Molecular survey of piroplasm species from selected area of China and Pakistan.” Parasites and Vectors 11. https://doi.org/10.1186/s13071-018-3035-x
Research and Development Projects
1. Development of a rapid and low cost lateral flow immunoassay (LFIA) diagnostic kit for Echinococcosis
With: Punjab Agriculture Research Board (2024-04 - 2027-03)
Working as Project Manager (Principal Investigator). Development of lateral flow diagnostic kit for Echinococcosis in cattle. The project is worth of PKR 16.471million
2. Screening of candidate antigens from Fasciola gigentica developmental stages, a way forward toward vaccine development
With: Punjab Agriculture Research Board (2022-10 - 2025-12)
Worked as Team Scientist in this project. The project worth was PKR 9.498 million. Selection of immunogenic antigen for vaccine development against fasciola gigantica
3. Mutation in Theileria annulata cytochrome b gene associated with buparvaquone resistance
With: HEC (2021-01 - 2022-01)
Worked as Principal Investigator. The project worth of PKR 1 million
The Islamia University of Bahawalpur was established in the year 1975 by upgrading historical institute of the Jamia Abbasia.
